By pushing throughput, single-cell transcript profiling can replace marker-based sorting and bulk RNA sequencing to redefine tissues from the bottom up.
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References
Jaitin, D.A. et al. Massively parallel single-cell RNA-seq for marker-free decomposition of tissues into cell types. Science 343, 776–779 (2014).
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Nawy, T. Single cells make the tissue. Nat Methods 11, 371 (2014). https://doi.org/10.1038/nmeth.2916
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DOI: https://doi.org/10.1038/nmeth.2916