Supplementary Figure 7: Chromatin recruitment experiments employing recombinant p300.
From: Accelerated chromatin biochemistry using DNA-barcoded nucleosome libraries
(a) Schematic representation of recombinant GST-tagged p300 constructs employed in this study. (b) N-terminally GST-tagged human p300 constructs were expressed recombinantly in E.coli and purified by glutathione affinity, ion exchange and size exclusion chromatography. Denaturing PAGE followed by CBB staining demonstrated > 95 % purity of the protein constructs. (c) Affinity pulldown of DNL-1 using resin-bound GST-p300-PHD. Experimental binding conditions and data processing were identical as in Fig. 2b. The input (IN)-normalized reads were averaged from 3 independent pulldown (PD) experiments and are expressed as (mean ± SD) employing the same grid as in Fig. 1b.
