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Genetics

Hitting the mark

Nature Methods volume 11, page 894 (2014)Cite this article

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Genome-editing technologies generally stay on target, but researchers should remain vigilant for variants acquired during experimental manipulation.

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References

  1. Smith, C. et al. Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs. Cell Stem Cell 15, 12–13 (2014).

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  2. Suzuki, K. et al. Targeted gene correction minimally impacts whole-genome mutational load in human-disease-specific induced pluripotent stem cell clones. Cell Stem Cell 15, 31–36 (2014).

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  3. Veres, A. et al. Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing. Cell Stem Cell 15, 27–30 (2014).

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Authors
  1. Michael Eisenstein
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Eisenstein, M. Hitting the mark. Nat Methods 11, 894 (2014). https://doi.org/10.1038/nmeth.3090

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