Supplementary Figure 10: Size-exclusion chromatograms of unsubstituted yeast (pre-)mRNPs. | Nature Methods

Supplementary Figure 10: Size-exclusion chromatograms of unsubstituted yeast (pre-)mRNPs.

From: Photo-cross-linking and high-resolution mass spectrometry for assignment of RNA-binding sites in RNA-binding proteins

Supplementary Figure 10

The right panel shows the chromatogram of a UV irradiated sample, the left panel a chromatogram of a non-irradiated control. Absorption at 254 nm (red) and 280 nm (blue) were monitored. Fractions and elution time are indicated on the bottom. Fractions #3–#5 were further processed for subsequent LC-MS/MS analysis. No absorbance differences in the chromatograms between control and cross-linked sample are observed as previously described by Urlaub et al.1

1. Urlaub, H., Kruft, V., Bischof, O., Muller, E.C. & Wittmann-Liebold, B. Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies. EMBO J 14, 4578-4588 (1995).

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