Supplementary Figure 11: Impact of N-peptidemut/nat on Env140ac– and Env140-nutR-H6ac–controlled EGFP expression in bacterial cells.
From: A general design strategy for protein-responsive riboswitches in mammalian cells
(a) Amino acid identity of native and mutated N-peptide (N-peptidenat/mut). (b) Flow cytometry-based histograms showing eGFP-mediated fluorescence correlating with the self-cleavage performance of Env140ac (pPST49) or Env140/nutR/H6ac (pPST106) in the presence of native (pPST102) or mutated (pPST171) N-peptide in E. coli. Red peak indicates co-transformation with native N-peptide, blue peak with mutated N-peptide.
