Supplementary Figure 1: Design of the HHR expression platform.
From: A general design strategy for protein-responsive riboswitches in mammalian cells
PmeI/SpeI restriction endonucleases are used for simple one-step swapping of the HHR scaffold among bacterial and mammalian expression vectors. Whereas the bacterial expression vector contains a PT7-driven eGFP expression unit (pET16b-eGFP) with the HHR scaffold embedded in the 5’ untranslated region, the mammalian expression vector harbors a PSV40-driven SEAP expression unit (pSEAP2-control) with the HHR scaffold inserted in the 3’ untranslated region.
