Supplementary Figure 8: DNA fragmentation and apoptosis in DRG and skin sections. | Nature Methods

Supplementary Figure 8: DNA fragmentation and apoptosis in DRG and skin sections.

From: Genetic targeting of chemical indicators in vivo

Supplementary Figure 8

(a) Lumbar DRG sections from Avil-Cre::SNAPCaaX mice injected with BG-Fluorescein into the hind paw, illuminated in vivo and subjected to the TUNEL assay 4 days after illumination. (b) DRG sections from whole mount Deleter-Cre::SNAPCaaX DRG labelled with BG-Fluorescein, illuminated ex vivo and subjected to the TUNEL assay. (c) Treatment of DRG sections with DNase I as an internal positive control for the TUNEL assay. Phase contrast images are shown below. (d) Skin sections from Avil-Cre::SNAPCaaX mice labelled with BG-Fluorescein, illuminated in vivo and subjected to the TUNEL assay 4 days after illumination. (e) Skin sections from Deleter-Cre::SNAPCaaX mice labelled with BG-Fluorescein, illuminated and subjected to the TUNEL assay. (f) Treatment of skin sections with DNase I as an internal positive control for the TUNEL assay. Scale bars 50μm.

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