Supplementary Figure 2: Deleter-Cre induces robust expression of membrane-associated Snap-tag. | Nature Methods

Supplementary Figure 2: Deleter-Cre induces robust expression of membrane-associated Snap-tag.

From: Genetic targeting of chemical indicators in vivo

Supplementary Figure 2

(a) Skin was isolated from mouse tail, labeled with SiR-SNAP for 30 minutes in PBS, fixed with 4%PFA for 20min, washed with PBS and then stained with Hoechst before confocal imaging. Negative control is shown in the lower panel. Scale bar, 20μm. (b) Brains were sectioned by microtome at a thickness of 300μm. Slices were labeled with SiR-SNAP in culture medium overnight, washed in PBS and fixed with 4%PFA for 20 minutes, washed in PBS again and stained with Hoechst before confocal image. Cortex is shown in the upper panel and negative control in the lower panel. Scale bar, 20μm. (c) Liver was sectioned by microtome at a thickness of 200μm. Slices were labeled with SiR-SNAP in PBS for 30min, fixed with 4%PFA for 20min, washed in PBS and stained with Hoechst before confocal imaging. Negative control is shown in the lower panel. Scale bar, 100μm. (d) Whole mount DRG were labeled with SiR-SNAP in culture medium overnight, washed in PBS and fixed with 4%PFA for 20min. Negative control is shown in the right panel. Scale bar, 100μm.

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