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Imaging

Transparency in large tissue samples

Nature Methods volume 12, page 11 (2015)Cite this article

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Recent improvements in tissue 'clearing' techniques permit their application to a variety of tissues and their combination with immunohistochemistry.

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References

  1. Renier, N. et al. iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell 159, 896–910 (2014).

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  2. Tainaka, K. et al. Whole-body imaging with single-cell resolution by tissue decolorization. Cell 159, 911–924 (2014).

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  3. Warner, C.A. et al. An optical clearing technique for plant tissues allowing deep imaging and compatible with fluorescence microscopy. Plant Physiol. 166, 1684–1687 (2014).

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Authors
  1. Nina Vogt
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Vogt, N. Transparency in large tissue samples. Nat Methods 12, 11 (2015). https://doi.org/10.1038/nmeth.3243

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