Supplementary Figure 6: Zoom-out of normalized frequency of nucleotides surrounding the rNMP sites

Normalized frequency of nucleotides relative to (a) nuclear and (b) mitochondrial mapped positions of sequences from ribose-seq library, PCR-amplified with EconoTaq DNA Polymerase (Lucigen), of genomic DNA from S. cerevisiae rnh201Δ (KK-100) cells. Position 0 corresponds to the rNMP. Negative and positive numbers (from -100 to -1 and 1 to 100) correspond to upstream and downstream positions from the rNMP, respectively. Frequencies were normalized to either nuclear or mitochondrial genomic mononucleotide frequencies. Normalized frequency of nucleotides relative to (c) nuclear and (d) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae rnh201Δ (KK-30) cells. Normalized frequency of nucleotides relative to (e) nuclear and (f) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae rnh1Δ rnh201Δ (KK-174) cells. Normalized frequency of nucleotides relative to (g) nuclear and (h) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae rnh1Δ rnh201Δ (KK-125) cells. Normalized frequency of nucleotides relative to (i) nuclear and (j) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae rnh1Δ rnh201Δ ung1Δ (KK-164) cells. Normalized frequency of nucleotides relative to (k) nuclear and (l) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae pol2-M644G rnh201Δ (KK-170) cells. Normalized frequency of nucleotides relative to (m) nuclear and (n) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae pol2-4 rnh201Δ (KK-107) cells. Normalized frequency of nucleotides relative to (o) nuclear and (p) mitochondrial mapped positions of sequences from ribose-seq library of genomic DNA from S. cerevisiae pol3-5DV rnh201Δ (KK-120) cells.