Supplementary Figure 1: Mechanism of alkaline cleavage of ribonucleotides in DNA
From: Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA
The ribonucleoside embedded in double-stranded DNA is in red. During alkaline treatment, DNA strands are denatured, and cleavage occurs at the rNMP site, generating a 2′,3′-cyclic phosphate end and an opposite 5′-hydroxyl end. The 2′,3′-cyclic phosphate is in equilibrium with 2′-phosphate and 3′-phosphate forms. Boxes in black indicate the 2′,3′-cyclic phosphate and 2′-phosphate DNA termini, which are substrates of AtRNL.
