Supplementary Figure 9: Specific proteases outperformed nonspecific proteases in disulfide-bond analysis of complex protein samples. | Nature Methods

Supplementary Figure 9: Specific proteases outperformed nonspecific proteases in disulfide-bond analysis of complex protein samples.

From: Mapping native disulfide bonds at a proteome scale

Supplementary Figure 9

(a) Number of disulfide bonds identified from an E. coli periplasmic fraction using different proteases. Number of unique identifications is shown in parentheses. (b) The search space and time cost for each protease digestion tested. Similar amounts of HCD spectra were collected from different protease digestions, and they were all searched against the same E. coli database using the same hardware setup (computer cluster, 6 units of Dual Xeon 5405, 2.0 GHz, 4G RAM, 8 core/unit) and the same pLink-SS parameters except for the protease specificity setting.

Back to article page