Supplementary Figure 1: Analysis of gene KO clones via T7E1 assay and DNA sequencing.
From: Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
(a) T7E1 assay confirming gene knockout in clonal populations of HAP1 haploid cells. WT, wild-type; MT, mutant; WT+MT, a 1:1 mixture of WT and MT PCR amplicons. (b) DNA sequences of wild-type and mutant clones. The PAM is shown in blue. Inserted bases are shown in red.
