Supplementary Figure 2: Analysis of off-target effects in gene KO clones via WGS. | Nature Methods

Supplementary Figure 2: Analysis of off-target effects in gene KO clones via WGS.

From: Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells

Supplementary Figure 2

(a) Small deletion in the ERBB3 KO clone confirmed by Sanger sequencing. (b) RGEN-mediated mutagenesis at the on-target and potential off-target sites. Mutation frequencies (%) were measured using T7E1 and targeted deep sequencing. (c) The on-target mutant sequence in the FGFR4 KO clone. The PAM sequence is shown in blue and inserted bases are shown in red. (d) Integrative Genomics Viewer (IGV) image at the FGFR4 on-target site.

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