Supplementary Figure 3: Examination of potential off-target sites.
From: Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
(a) The number of potential off-target sites that differ from on-target sites by up to 8 nucleotides or by 2 nucleotides with a DNA or RNA bulge of up to 5 nucleotides in length. (b) Schematic of consensus sequence generation. (c) On-target mutations in five KO clones identified by consensus sequence comparison.
