Supplementary Figure 5: An overview of map features necessary for accurate structure determination. | Nature Methods

Supplementary Figure 5: An overview of map features necessary for accurate structure determination.

From: Atomic-accuracy models from 4.5-Å cryo-electron microscopy data with density-guided iterative local refinement

Supplementary Figure 5

(a) The 20S proteasome dataset at 4.1 Å nicely illustrates the necessary features for structure determination to atomic accuracy. The pitch of helices and individual strands are resolved, and – while sidechains are not uniquely identifiable – density for large sidechains is visible. (b) The same dataset at 6 Å resolution lacks these features: the pitch of helices in not identifiable, and sidechain density for large sidechains is not observed.

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