Supplementary Figure 13: MinION data for full-length (48-kb) λ phage dsDNA.

Data for a 2D read of a full-length λ phage dsDNA from the MinION. (a) Molecular events for translocation of a single 48-kb λ dsDNA molecule through the MinION nanopore sequencer. DNA length and conformation are simplified for purposes of illustration. (i) Open channel. (ii) dsDNA with ligated loading (blue and brown) and hairpin adaptors (red) captured by the nanopore with the aid of a membrane anchor and an applied voltage across the membrane. (iii) Translocation of the 5′ end of the loading adaptor through the nanopore under control of a molecular motor and driven by the applied potential across the membrane. DNA translocation through the nanopore starts. (iv) Translocation of the template strand of DNA (gold). (v) Translocation of the hairpin adaptor (red). (vi) Translocation of the complement strand (blue). (vii) Translocation of the 3′ portion of the loading adaptor. (viii) Return to open-channel nanopore. (b) Raw current trace for the entire passage of the DNA construct through the nanopore (approximately 2,789 s). Regions of the ionic current trace corresponding to steps i–viii are labeled. (c) Expanded 1-s time scale of raw current traces for DNA capture and translocation of 5′ loading adaptors (i–iii), template strand (iv), hairpin adaptor (v), complement strand (vi), 3′ loading adaptor and return to open channel (vii–viii). Each adaptor generates a unique signal used for position reference in base determination. The FASTA sequence is available at http://figshare.com/articles/UCSC_Full_Length_Lambda_2D_Read/1209636.