Supplementary Figure 2: Venn diagram representing read mappability for MinION reads across three replicate M13 experiments using R7.3 chemistry.

Mappability represents the proportion of reads that can be aligned to either the M13 or the phage λ DNA control using the tuned parameters for each mapper. In our analysis, 2D reads had the highest mappability, with 99% of reads being mappable, followed by complement and template reads, with 98% and 95% of their respective read proportions being mappable. Among the four aligners used, LAST and LASTZ performed the best for M13, with LAST capturing the greatest proportion of mappable reads on its own.