Supplementary Figure 1: ROTO staining and ultrastructural preservation over different depths.
From: High-resolution whole-brain staining for electron microscopic circuit reconstruction
SEM images for ROTO-prepared samples without, (a-e), and with, (f-i), extracellular space (ECS) preservation. Locations for high-resolution images indicated in the corresponding low-resolution scans. Imaging parameters are 440 nm pixel size, 2.8 kV, 100 pA, and 0.0064 e-/nm2 in a and f, 5 nm pixel size, 2.8 kV, 300 pA, and 600 e-/nm2 in b-e, and 10 nm pixel size, 2.8 kV, 100 pA, and 100 e-/nm2 in g-i. Scales bars are 100 μm in a and f, 250 nm in b-e and 500 nm in g-i.
