Supplementary Figure 5: Crack in BROPA sample.
From: High-resolution whole-brain staining for electron microscopic circuit reconstruction
(a) Cracks, generally devoid of epoxy, are occasionally observed in epoxy-embedded BROPA samples (see Supplementary Video 4). (b) High-magnification image of the red asterisk in a. Imaging parameters are 220 nm pixel size, 3.0 kV and 0.29 e-/nm2 in a and 10 nm pixel size, 3.0 kV and 140 e-/nm2 in b. Scale bars are 100 μm in a and 5 μm in b.
