Supplementary Figure 3: Segmentation accuracy in different biological systems acquired with multiple microscopy setups
From: In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy
The datasets in (a)-(c) were kindly provided by Philipp Keller and were also analyzed by his group in Fig. 3 of Amat et al., 201420. (a) Segmentation analysis of a stage 6 Drosophila embryo expressing His2Av-mRFP1 acquired on a Zeiss Lightsheet Z.1 microscope (timepoint 500 of the dataset), (b) a zebrafish embryo during early gastrulation expressing H2B-mCherry acquired on a Zeiss LSM 710 laser-scanning microscope (timepoint 0 of the dataset) and (c) a mouse embryo expressing H2B GFP acquired on a SimView light sheet microscope (timepoint 0 of the dataset) (d)-(f) Segmentation results for cancer cell line spheroids grown from (d) HT-1080, (e) T47D and (f) MCF7 cells and expressing H2B GFP. The LoG response thresholding and the local signal to background ratio thresholding algorithms provided in the software were chosen to obtain the initial foreground-background segmentation for the datasets in panels (a) and (b-f), respectively.
