Supplementary Figure 11: Multiview imaging of Drosophila embryo.
From: 3D high- and super-resolution imaging using single-objective SPIM
(a) soSPIM optical sections of a Drosophila embryo expressing the nuclear protein histone-mCherry imaged with a 20×/0.5-NA objective and a 4.3-μm-thick light sheet for three different rotation angles of the embryo along the 45° mirror. (b) Schematic representation of the soSPIM multiview system. The embryo is inserted inside a UV-curable index-matched capillary polymer that rotates along a millimeter-sized mirror allowing multiview imaging of the embryo (Supplementary Fig. 5b and Online Methods). Scale bar, 50 µm.
