Supplementary Figure 8: Line scanning reveals the peak primed conversion efficiency that can be achieved in vitro.
From: In vivo single-cell labeling by confined primed conversion
In this experiment, Dendra2 was photoconverted in a similar manner to Supplementary Fig. 7: the priming beam power was either a, b, varied while scanning repetitions were fixed, or c, d, fixed while scanning repetitions were varied. The major difference in the experiment was that photoconversion was achieved with a line scan (512 x 1 pixels). Photoconversion (maximum is ~ 60-fold) at a, a fixed number of iterations (2400 repetitions) and c, a fixed priming beam power (0.02 mW). b, d, Photobleaching of the green form of Dendra2 after laser illumination. For each pair (primed conversion versus 488 nm alone), a two-tailed, unequal variance t-test was performed: *, P < 0.05; **, P < 0.005.
