Supplementary Figure 9: Schematic illustration of how the conversion filter plate confines primed conversion to the focus.
From: In vivo single-cell labeling by confined primed conversion
The conversion filter plate is placed in the infinity space of the beam path where the lasers (represented as individual ray beamlets) are collimated, within a filter cube beneath the objective. Each half of the conversion filter plate passes either the priming beam or the converting beam. Thus, the lasers only meet within the sample at the focus. An opaque strip aids in separating the beams, maintaining axial confinement by ensuring a sufficient time delay between both lasers as they scan areas above and below the focus.
