Supplementary Figure 10: Confinement of primed conversion is most efficient when a region of interest is irradiated using a short pixel dwell time.
From: In vivo single-cell labeling by confined primed conversion
Left column: axial (XZ) average intensity projection images of the photoconverted red Dendra2 channel show the confinement of Dendra2 photoconversion as a function of pixel dwell time (12.61 µsec in a, 2.55 µsec in b, and 0.64 µsec in c). Right column: These graphs show the normalized intensity profiles of the images in the left column. The areas outside of the approximated full width half maximum (FWHM) are grayed out. Confinement of photoconverted red Dendra2 signal in a, is ~ 4-5 µm, b, is ~ 6.5 µm, and c, is nearly 10 µm. In each case, the level of red signal decreases sharply to just above the background level (compare to Fig. 1g in the main text, without the conversion filter plate in place). Scale bar, 5 µm (lateral dimension) and 2 µm (axial dimension).
