Supplementary Figure 12: Regardless of the location in the zebrafish, cells can be individually labeled using spatially confined primed conversion.

These panels show the individual green (left column) and red (magenta pseudocolor, right column) channels for the images from Fig. 2 e, f, which were taken within 3 dpf zebrafish expressing neural specific Dendra2. a, c, Individual cell projections become indiscernible within clusters of a, synapsing neurons in the optic tectum and c, among parallel bundled neural tracts within the developing spinal territory, which can be seen in the green Dendra2 channel. b, d, We used primed conversion to select out a cell of interest in these regions of the brain (arrowheads), and the photoconverted Dendra2 quickly diffused out from the cell body, allowing us to uncover the neurites within these cells. Scale bars, 20 µm.