Supplementary Figure 1: Two-photon (2P) photoconversion is negligible for many popular photoconvertible proteins.
From: In vivo single-cell labeling by confined primed conversion
a,b, After strong (50 mW average power) illumination, the red channel data (a) shows photoconversion for many different FPs (< 3 fold increase over background). The green channel (b) shows photobleaching. As a control, we used the same settings to try to photomodulate the dark-to-red photoactivatable FP PAmCherry (black data points). The dashed line in a indicates the fold change possible with primed conversion (see Fig. 1d). c, d, Comparison of long-term (4.5 min) 2P illumination (white bars) versus 405 nm photoconversion (gray bars) for all tested green-to-red photoconvertible proteins. As before, the red channel data (c) shows photoconversion, while the green channel data (d) shows photobleaching. In the green channel data, a value of 1 indicates that the green population is unaffected by pulsed illumination. For each pair in c, d, a t-test (two-tailed, unequal variance) was performed: c, *P ~ 0.005, **P < 0.005; d, *P < 0.005.
