Supplementary Figure 2: In-depth characterization of Dendra2 confirms negligible photoconversion with 2P laser sources.
From: In vivo single-cell labeling by confined primed conversion
Green Dendra2 was irradiated with focused light from a tunable 2P laser source at different wavelengths and illuminating powers (25 mW, dark gray bars; 50 mW, gray bars; 75 mW, white bars) for either a, b, a short exposure time or c, d, an approximately 8-fold longer exposure time (see Online Methods). After laser illumination, the red channel data in a, c, show photoconversion is at most 5-fold above background, and b, d, green channel data show significant photobleaching at higher power illumination (fold change is less than 1). At each wavelength, a one-way ANOVA test was performed: a, P < 0.05 for only the 800 nm case; b, P < 0.01 for all wavelengths; c, P < 0.05 for 750, 850, and 900 nm; d, P < 0.05 for all wavelengths. * represents significance between a pair within a 95% confidence interval and ** represents significance between a pair within a 99% confidence interval after post-hoc analyses (see Online Methods).
