Supplementary Figure 5: Photoconversion with either 405-nm illumination or primed conversion results in a photoconverted Dendra2 population with the same spectral properties and photostability.
From: In vivo single-cell labeling by confined primed conversion
a, The excitation and b, emission spectra (normalized to the peak value) of photoconverted red Dendra2 after primed conversion (black curve) and after near-UV illumination (gray curve). In the graphs, a reference spectrum (red dashed curve) for the photoconverted form of Dendra2 is also shown (see Online Methods). c, Dendra2 was continuously exposed to 561 nm light for ~290 seconds. Individual frames from this time-lapse were analyzed and fit to a bi-exponential function (see Online Methods). d, Samples of photoconverted Dendra2 that were kept in the dark between imaging experiments were imaged at several time points (see Online Methods) to characterize the chemical stability of the photoconverted form of the FP. The half life of the red form of Dendra2 in vitro is approximately 4 days. For each time point in (c) and (d), a t-test (two-tailed, unequal variance) was performed: differences between the two photoconverted Dendra2 populations were not significant (P > 0.1) for all time points.
