Supplementary Figure 7: Efficient primed conversion is achieved using moderate imaging conditions.
From: In vivo single-cell labeling by confined primed conversion
Dendra2 was photoconverted either with a, b, varying priming beam power and a constant number of scan repetitions or c, d, constant priming beam power and a varying number of scan repetitions. In all cases, the converting beam was fixed at 5 mW. Photoconversion was achieved by scanning an entire frame (512 x 512 pixels) within the embedded protein gel (see Online Methods). a, c, Photoconversion fold change in the red channel for primed conversion (gray bars) and for 488 nm illumination (< 6-fold, white bars). b, d, Photobleaching of the unphotoconverted green Dendra2 population after laser illumination. For each pair (primed conversion versus 488 nm alone), a two-tailed, unequal variance t-test was performed: *, P < 0.05; ** P < 0.005.
