Supplementary Figure 1: Confirmation of the Ar mutation from the analysis of whole-genome sequencing | Nature Methods

Supplementary Figure 1: Confirmation of the Ar mutation from the analysis of whole-genome sequencing

From: Off-target mutations are rare in Cas9-modified mice

Supplementary Figure 1

The raw PINDEL output (top panel) and pileup of supporting sequence reads (lower panel) for F1 animal F18-9 shows a heterozygous deletion of 58 bp in exon 1 of Ar, previously characterized in founder animal F18 by PCR sequencing2. Note the fall in read coverage across the region and the mapping of soft-clipped reads.

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