Supplementary Figure 3: Alignment of Ar sgRNAs to high-quality variants do not correspond to a bona fide off-target site | Nature Methods

Supplementary Figure 3: Alignment of Ar sgRNAs to high-quality variants do not correspond to a bona fide off-target site

From: Off-target mutations are rare in Cas9-modified mice

Supplementary Figure 3

The PAM site in the genomic sequence is highlighted in red text. (a) Ungapped alignment showing 7 mismatches between the sgRNA and genome sequence. The variant sequence is located 9 bp upstream of the predicted Cas9 cleavage site (arrowhead). The 2bp deletion detected at this site (Chr10:39516743) in animal F18-6 is underlined. (b) Gapped alignment showing 4 mismatches. The variant lies outside of the target sequence, 8 bp downstream of the predicted Cas9 cut site (arrowhead). The 6 bp deletion detected at this site (Chr3:141956378) in animals F25-5 and F25-6 is underlined.

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