Supplementary Figure 6: AFM topograph and multiparametric maps of PAR1 reconstituted in liposomes.
From: Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
(a) Topograph showing single and clustered PAR1 molecules protruding from the lipid bilayer. (b) Applied force error map showing low errors of <20 pN. (c) Adhesion map showing the SFLLRN functionalized AFM tip interacting sparsely with the lipid bilayer and mainly with PAR1 (see (a)). (d) Deformation map showing enhanced deformation values of PAR1 molecules. The FD-based AFM data was recorded as described (Online Methods ).
