Supplementary Figure 7: Mapping the binding of the SFLLRN ligand to human PAR1 proteoliposomes using FD-based AFM.
From: Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
Topographs (left column) of human PAR1 reconstituted in proteoliposomes taken with the SFLLRN-ligand functionalized AFM tip oscillated at 0.25 kHz and amplitude of 50 nm. As described, the SFLLRN has been attached via the PEG-polypeptide linker to the AFM tip (Fig. 1c). Corresponding adhesion maps (right column). To increase their visibility adhesive pixel were enlarged by a factor 2. The FD-based AFM data was recorded as described (Online Methods ).
