Supplementary Figure 8: Validating that SFLLRN ligand–functionalized AFM tips detect specific interactions with PAR1.
From: Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
Height images (a,c,e,g) and corresponding adhesion images (b,d,e,h) recorded with either (a,b) a bare AFM tip, (c,d) a hexa-glycine, (e,f) a scrambled peptide (FLLNSR) or (g,h) a SFLLRN functionalized tip in the presence of peptide mimetic antagonist (1 µM BMS). Every functional group (hexa-glycine, FLLNSR, or SFLLRN) was attached via the same PEG-polypeptide linker to the AFM tip (Fig. 1c). This supplementary figure correlates to Fig. 2, which maps receptor-ligand interactions on PAR1 using SFLLRN functionalized AFM tips. The FD-based AFM data was recorded as described (Online Methods ).
