Supplementary Figure 10: Validating the elastic potential of the PEG-polypeptide linker attaching the ligand to the AFM tip.
From: Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
Shown are individual FD curves, each one detecting the rupture of a specific SFLLRN-PAR1 bond. Each of these representative FD curves was fitted using the effective spring constant keff of the cantilever-PEG-polypeptide linker system (Supplementary Fig. 9). The individual fits were shifted along the distance axis to minimize the sum of squared residuals between the fit and the adhesion force peak. The ∆d values given correspond to the shifted distance. FD curves were recorded as described (Fig. 2) using a SFLLRN functionalized AFM cantilever and imaging a PAR1 proteoliposome by FD-based AFM.
