Supplementary Figure 2: SDS-PAGE of PAR1 liposomes.
From: Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
SDS-PAGE was performed on a 12% acrylamide gel with 150 V and 60 min in a loading buffer (50 mM Tris, pH 7, 6% SDS, 50 mM DTT, 8% glycerol, 0.1% bromphenol blue) and stained with Coomassie blue. Lane 1, molecular weight marker; Lane 2, 10 µL of empty lipid vesicles (liposomes made of 0.5 mg mL−1 DOPC and 0.05 mg mL−1 CHS); Lane 3, 10 µL of PAR1 proteoliposomes (10 µM PAR1 reconstituted in liposomes made of 0.5 mg mL−1 DOPC and 0.05 mg mL−1 CHS). The band at ≍40 kDa is in good agreement with the expected size of 43.9 kDa calculated from the PAR1 sequence.
