Supplementary Figure 3: Theoretical unfolding transition behaviors.
Temperature – fluorescence unfolding curves of a theoretical three-subunit complex are shown. Left: The single-transitions of the individual components are depicted. Right: Graphs depict a normalized sum of three individual curves. (a) The three components unfold independently from each other at different temperatures. The sum is a curve showing several independent transitions. (b) The components unfold at similar temperatures but still independently from each other. The resulting sum resembles a two-state unfolding curve. (c) The components unfold cooperatively at the same temperature. This also results in a two-state curve, which is steeper than in the middle case. However, the melting temperature of the shallower uncooperative unfolding transitions results in an apparently higher melting point Tm1, than the cooperative transition (Tm2) as visualized by the dotted lines.
