Supplementary Figure 13: Locus-specific validation of 5fC sites.
From: Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
(a,b) fCAB-Seq8 and MAB-Seq13 were performed (in parallel with fC-CET) on the 76-mer 5fC model sequence. Results from TOPO-cloning assay (a) and high-throughput sequencing (b) are shown. In both cases, a normal C on the 5fCpG nearby was used as a control. (c–e) Locus-specific validation of 5fC sites with fC-CET (c,d) and fCAB-Seq (e). The columns represent the percentage of T at the target 5fC sites. For each site, a P value is given to show whether it is a statistically significant 5fC site (binomial test for c and d, and Fisher’s test for differences between fCAB and BS datasets in e). *P < 0.05, **P < 0.01, ***P< 0.001; #, not detected because of technical issues.
