Supplementary Figure 1: Chemical labeling of 5fC via Friedländer reaction.
From: Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
(a) Proposed chemical labeling of 5fC based on the principle of Friedländer reaction30. (b) Chemicals screened for 5fC labeling, with reaction yield indicated. 1, ethyl acetoacetate (EA). 2, methyl acetoacetate (MA). 3, ethyl 6-azido-3-oxohexanoate (EAO). 4, diethyl malonate (DM). 5, 1,3-Indandione (I). 6, 5-(2-azidoethyl)-1, 3-indandione (AI). 7, ethyl nitroacetate. 8, ethyl cyanacetate. 9, acetylacetone. 10, benzoylacetone. 11, barbituric acid. 12. benzoylacetonitrile. n.d., no detectable products as measured by MALDI-TOF mass spectrometry.
