Supplementary Figure 3: HPLC analysis of 1,3-indanedione labeling on 5fC-containing 9-mer model DNA.
From: Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
9-mer 5fC and 5fC-I oligonucleotides were digested to nucleosides and analyzed with a C-18 column of HPLC (260 nm and 310 nm). The peak for 5fC was completely undetectable after the reaction, indicating full labeling. Authentic 5fC or 5fC-I nucleosides were also analyzed as positive controls.
