Supplementary Figure 1: Data normalization.
From: Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling
Average thermal profiles are plotted for DMSO- and (S)-crizotinib-treated cells. Note that the two profiles (broken straight lines) are similar (no thermal shift) but imperfect. The two fitted sigmoid models are very close (dashed smooth lines), thus further indicating the absence of influence of (S)-crizotinib on the average thermal stability. Vertical arrows (only displayed for the DMSO sample for simplicity), expressed as shift in normalized coordinates, can be applied to spectra specific to a given protein for subsequent scoring of the whole dataset and can be regarded as a structural normalization considering the different protein content at each temperature.
