Supplementary Figure 13: The relative stoichiometry of the cell compartment–specific exosome complexes.
From: A strategy for dissecting the architectures of native macromolecular assemblies
The SDS-PAGE gels of the Rrp6- and Ski7-associated exosome complexes were stained with Sypro Ruby. The proteins were visualized using an LAS-3000 Fujifilm system, and the corresponding intensities of the four protein bands were measured by ImageJ to calculate their stoichiometries relative to Rrp44. The error bars represent standard deviations from four independent experiments. The gel band at 20 kDa corresponds to both Lrp1 and Mpp6. However, because Lrp1 is the dominant protein in the band, the protein intensity of Mpp6 was neglected for the present stochiometric analysis of Rrp6-associated, nucleus-specific exosome complex.
