Supplementary Figure 2: Tissue array cell-seeding method optimization.
From: Tissue matrix arrays for high-throughput screening and systems analysis of cell function
(a) Tissue microarrays were designed to randomize the location of each type of tissue spot to limit error associated with cell seeding. When the two spot patterns shown are used in duplicate (n = 6 array spots per tissue type), each spot type is distributed between the inner and outer columns and inlet and outlet rows. (b) A polydimethylsiloxane (PDMS) compartment was designed to confine medium flow and promote uniform cell seeding. (c) Variability associated with cell seeding in PDMS compartments on control microarray chips (all spots soluble collagen control) was less than 15% between rows and columns (coefficient of variation, n = 10 rows, n = 4 columns, n = 2 array chips). Data are mean.
