Supplementary Figure 8: ChIP-qPCR of H3K9 trimethylation at the HS2 enhancer. | Nature Methods

Supplementary Figure 8: ChIP-qPCR of H3K9 trimethylation at the HS2 enhancer.

From: Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

Supplementary Figure 8

(a) ChIP-seq tracks show increased H3K9me3 signal at the HS2 enhancer (shaded area, magnified inset). An ENCODE K562 DNase I hypersensitivity DNase-seq track is included to highlight the globin LCR49. Two primer sets were designed for the HS2 enhancer for ChIP-qPCR of H3K9me3. (b) ChIP-qPCR demonstrates increased H3K9me3 when dCas9-KRAB was targeted to the HS2 enhancer (mean ± s.e.m., n = 3 independent experiments).

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