Supplementary Figure 3: Protein silencing of HBG1 by dCas9-KRAB transcription factors targeted to the distal HS2 enhancer. | Nature Methods

Supplementary Figure 3: Protein silencing of HBG1 by dCas9-KRAB transcription factors targeted to the distal HS2 enhancer.

From: Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

Supplementary Figure 3

Western blot for γ-globin and GAPDH demonstrate globin silencing in K562 cells treated with dCas9-KRAB (dCK) and sgRNAs compared to non-transduced controls (No LV), no sgRNA controls, IL1RN-targeted sgRNA controls, and dCas9 (dC) with sgRNA (cropped from representative images from n = 3 biological replicates). Western blot for the FLAG epitope show dCas9 and dCas9-KRAB expression in transduced K562 cells.

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