Supplementary Figure 1: Targeted extraction and statistical validation of ion chromatograms (TeXaS). | Nature Methods

Supplementary Figure 1: Targeted extraction and statistical validation of ion chromatograms (TeXaS).

From: xTract: software for characterizing conformational changes of protein complexes by quantitative cross-linking mass spectrometry

Supplementary Figure 1

(a) Workflow of the TeXaS algorithms developed to quantify and validate ion chromatograms from cross-linked peptides from data dependent analysis MS experiments. (b) Model of an extracted ion chromatogram of a cross-linked peptide ion showing the extracted ion chromatograms of the individual isotopes. (c) Statistics on all extracted ion chromatogram peak groups generated by mProphet. (d) Sensitivity and q-value vs. the normalized discriminant score. (e) Sensitivity and q-value. The data point at which the sensitivity reaches its maximum is indicated by the dot. This corresponds to the maximal q-value that is used to further process the peak groups.

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