Supplementary Figure 3: Histogram of measured Cα distances of cross-links from the dilution series dataset. | Nature Methods

Supplementary Figure 3: Histogram of measured Cα distances of cross-links from the dilution series dataset.

From: xTract: software for characterizing conformational changes of protein complexes by quantitative cross-linking mass spectrometry

Supplementary Figure 3

Validation of the cross-links from the dilution series dataset of model proteins on the available protein structures or homology models of BSA, transferrin and conalbumin (see also Online methods). Out of 270 cross-links that were mapped onto the structures from the different workflows 12 cross-links exceeded the distance threshold of 32 Å (blue). This corresponds to 4.4% of all validated cross-links and is in good agreement with the selected FDR cutoff of 5%.

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