Supplementary Figure 15: Comparison of single-view and IsoView deconvolution (developmental imaging 2).
From: Whole-animal functional and developmental imaging with isotropic spatial resolution
Comparative visualization as in Supplementary Fig. 14, but for y-z image cross-sections taken from image data of a gastrulating Drosophila embryo (Supplementary Video 5). The lower block of image panels (“Region 5”) exemplifies a worst-case scenario for four-view imaging: membranes and cell nuclei are oriented close to a 45-degree angle relative to the microscope’s two principal imaging axes (y- and z-axes). Thus, for the particular sample geometry encountered here, the four views of the IsoView microscope provide less high-frequency content than available for any other sample geometry (see also Swoger et al. 2007, Optics Express). The corresponding y-z image cross-sections illustrate that IsoView is nevertheless capable of substantial improvements in overall resolution and resolution isotropy relative to conventional imaging, resolving sub-cellular features such as plasma membranes also in this challenging scenario. Scale bar, 10 µm.
