Supplementary Figure 2: IsoView two-color imaging.
From: Whole-animal functional and developmental imaging with isotropic spatial resolution
IsoView schematic as shown in Supplementary Fig. 1, but indicating the two different wavelength configurations used for rapid four-view, two-color imaging in IsoView mode 3 (Fig. 1d). The microscope quickly alternates between configurations (a) and (b), in which the specimen is illuminated with two different laser wavelengths and two different spectral bands are imaged along orthogonal axes. Color assignments in the four illumination and detection arms are flipped in orthogonal arms when switching between configurations (a) and (b). Completing a full volumetric imaging cycle with both wavelength configurations produces four-view image data sets for both spectral channels.
